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A high-yield outbred suckling mouse model of cryptosporidiosis
M Buraud1, N Kapel, Y Benhamou
1Service de Biologie Animale et Parasitaire, UFR des Sciences Pharmaceutiques et Biologiques, Université René Descartes, Paris.
Abstract:
Outbred suckling mice (NMRI strain) were used as hosts. They were initially inoculated with oocysts of human origin, and subsequently with parasites recovered from the mouse ileal mucosa. Cryptosporidia were counted in an aliquot of whole-ileum homogenate. Parasite load was expressed as cryptosporidia per centimeter of ileum. Serial passage of C. parvum in NMRI mouse litters led to a gradual amplification of parasite burden relative to animals initially inoculated with the human isolate.
Insights
Serial passage of Cryptosporidium parvum in mice amplified parasite burden. This study demonstrates increased C. parvum load after repeated host passage in NMRI mice.
Area of Science:
- Veterinary Parasitology
- Infectious Diseases
- Microbiology
Background:
- Cryptosporidium parvum is a significant enteric pathogen.
- Understanding host-parasite dynamics is crucial for disease control.
Purpose of the Study:
- To investigate the effect of serial passage on Cryptosporidium parvum infectivity and parasite burden in a mouse model.
- To establish a model for studying Cryptosporidium amplification.
Main Methods:
- Outbred NMRI mice were inoculated with human-origin Cryptosporidium oocysts.
- Parasites were serially passaged through mouse litters using ileal mucosa isolates.
- Parasite load was quantified as cryptosporidia per centimeter of ileum.
Main Results:
- Serial passage led to a gradual amplification of parasite burden.
- Mice receiving passaged parasites exhibited higher loads compared to initial inoculation.
- NMRI mice supported increasing Cryptosporidium loads with serial passage.
Conclusions:
- Serial passage of Cryptosporidium parvum in NMRI mice effectively amplifies parasite burden.
- This model can be utilized for further research into Cryptosporidium pathogenesis and treatment.
- Host adaptation and amplification are key factors in Cryptosporidium infections.