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Updated: Aug 12, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Two pathways of the nitric oxide-induced cytotoxycal action
K T Turpaev1, A M Amchenkova, A N Narovlyansky
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. turpaev@imb.imb.ac.ru
Abstract:
Nitric oxide is a diffusible messenger with multiple biological functions. We show here that NO-generating compound, S-nitrosoglutathione (GSNO) induces apoptosis in human chondrocytes and causes necrosis-like cell death in human epithelial CaOv cell line. Pretreatment of chondrocytes with low-dose GSNO or with gamma-interferon enhances their tolerance to the second high-concentration GSNO exposure. On the contrary, in CaOv cells low-dose GSNO pretreatment diminishes the resistance and increases cytolysis at the second GSNO exposure. We conclude that human chondrocytes possess specific and inducible mechanism preventing cell killing by nitric oxide.
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