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Related Experiment Videos

Basic FGF regulates interstitial collagenase gene expression in human smooth muscle cells

S H Kennedy1, S Rouda, H Qin

  • 1Department of Pathology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.

Journal of Cellular Biochemistry
|April 1, 1997
PubMed
Summary

Basic fibroblast growth factor (bFGF) increases interstitial collagenase expression in human vascular cells. This promotes extracellular matrix turnover, potentially driving smooth muscle cell proliferation in atherosclerosis and restenosis.

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Area of Science:

  • Molecular Biology
  • Cardiovascular Research

Background:

  • Basic fibroblast growth factor (bFGF) is a key mitogen involved in vascular smooth muscle cell growth.
  • Atherosclerosis and vascular restenosis are characterized by abnormal smooth muscle cell proliferation and extracellular matrix remodeling.

Purpose of the Study:

  • To investigate the effect of bFGF on interstitial collagenase gene expression in human vascular smooth muscle cells.
  • To elucidate the molecular mechanisms underlying bFGF-induced collagenase regulation.

Main Methods:

  • Northern blot analysis to quantify collagenase mRNA levels.
  • RT-PCR to assess pre-mRNA levels.
  • Reporter gene assays (promoter-CAT) to evaluate transcriptional activity.
  • mRNA degradation studies.

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  • Western blot analysis to detect collagenase protein.

    • Main Results:

    • bFGF significantly increased collagenase mRNA and pre-mRNA levels within 24 hours.
    • Reporter gene assays indicated that bFGF did not upregulate the main collagenase promoter but modestly stimulated specific promoter fragments.
    • bFGF did not affect collagenase mRNA stability.
    • Increased collagenase mRNA correlated with elevated collagenase protein levels.

    Conclusions:

    • bFGF upregulates interstitial collagenase expression at a post-transcriptional or translational level.
    • This upregulation leads to extracellular matrix turnover, potentially contributing to smooth muscle cell migration and proliferation in cardiovascular diseases.
    • The findings highlight bFGF's role in matrix remodeling relevant to atherosclerosis and restenosis.