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Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
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Feulgen staining of intact plant tissues for confocal microscopy

J P Braselton1, M J Wilkinson, S A Clulow

  • 1Department of Environmental and Plant Biology, Ohio University, Athens, 45701-2979, USA.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|March 1, 1996
PubMed

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...