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Plant cuticle staining with bismarck brown Y and azure B or toluidine blue O before paraffin extraction

E T Graham1, P A Joshi

  • 1Department of Ornamental Horticulture, The University of Tennessee, Knoxville, 37901-1071, USA.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|March 1, 1996
PubMed

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...