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The use of polymerase chain reaction (PCR) as a diagnostic tool for dengue virus
R Thayan1, B Vijayamalar, S Zainah
1Division of Virology, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
The Southeast Asian Journal of Tropical Medicine and Public Health
|December 1, 1995
Abstract:
This study describes the use of polymerase chain reaction as a diagnostic tool for detecting and typing of dengue virus. PCR was compared against virus isolation. First RT-PCR was done using dengue consensus primers after which positive samples were subjected to RT-PCR using type-specific primers. This study shows that the local strains of the dengue virus could be detected using the chosen primers. Furthermore, RT-PCR was found to be more sensitive than virus isolation in identifying the dengue positive samples.