[Gel filtration of protected synthetic peptides in dimethylformamide]

For large water-insoluble protected peptides, a novel method of analytical and preparative size exclusion liquid chromatography on Toyopearl HW-40, HW-50, HW-55, and HW-60 gels was proposed, with DMF as an eluent. The selectivity and molecular mass ranges of these gels were determined for separation of protected peptides in DMF. The molecular mass ranges of Toyopearl HW gels for protected peptides in DMF were found to differ significantly from those for peptides and proteins in aqueous buffer solutions.