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A novel Arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases
K Gutshall1, K Wang, J E Brenchley
1Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
Journal of Bacteriology
|May 1, 1997
Abstract:
An Arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a Xanthomonas manihotis enzyme. Phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade. The gene encodes a subunit of 52 kDa, and the enzyme appears to be active as a dimer. The enzyme hydrolyzed substrates with either a beta-1,4 or a beta-1,3 linkage.