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In vitro formation of crocetin glucosyl esters by Crocus sativus callus extract
C Dufresne1, F Cormier, S Dorion
1Food Research and Development Centre, Agriculture and Agri-Food Canada, St-Hyacinthe, Québec, Canada.
Planta Medica
|April 1, 1997
Abstract:
Callus extracts of Crocus sativus exhibited the ability to transform all-trans-crocetin into its related glycosides between pH 7.0 and 7.6 in the presence of uridine-diphospho-glucose. The reactions involved the step-wise addition of a glucose moiety to a free carboxyl function and the 1-->6 addition of a glucose moiety to a glucosyl ester function. The kinetics of synthesis for each glycoside seemed to indicate that two distinct glucosyl transferases were implicated in the synthesis of crocin, all-trans-crocetin di-(beta-D-gentiobiosyl) ester.