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Related Experiment Videos

ATP-activated cationic current in rabbit sino-atrial node cells

M Shoda1, N Hagiwara, H Kasanuki

  • 1Heart Institute of Japan, Tokyo Women's Medical College, Japan.

Journal of Molecular and Cellular Cardiology
|February 1, 1997
PubMed
Summary

Extracellular adenosine triphosphate (ATP) activates a unique ion current in rabbit heart cells. This ATP-activated current, likely mediated by P2-purinoreceptors, may influence heart rate regulation.

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Area of Science:

  • Cardiovascular Physiology
  • Electrophysiology
  • Purinergic Signaling

Background:

  • Autonomic nerve endings release transmitters in the heart, influencing heart rate.
  • Extracellular adenosine triphosphate (ATP) is a key signaling molecule in the cardiovascular system.
  • The specific ion channels activated by extracellular ATP in sino-atrial node cells are not fully characterized.

Purpose of the Study:

  • To investigate the electrophysiological effects of extracellular ATP on rabbit sino-atrial node cells.
  • To characterize the properties and ionic selectivity of the ATP-activated current.
  • To explore the potential role of this current in cardiac chronotropy.

Main Methods:

  • Voltage-clamp recordings from single rabbit sino-atrial node cells.

Related Experiment Videos

  • Application of varying concentrations of extracellular ATP and its analogs.
  • Measurement of ion permeability using reversal potential analysis.
  • Assessment of channel blocker and anion substitution effects.

    • Main Results:

    • Extracellular ATP (0.01-1 mM) activated a time-independent, weakly inwardly rectifying current.
    • The current exhibited dose-dependent activation by ATP but not by non-hydrolyzable analogs or other adenosine derivatives.
    • The ATP-activated current showed non-selective permeability to monovalent cations, including large ions like N-methyl-D-glucamine.
    • The current was independent of chloride ions.

    Conclusions:

    • A novel ATP-activated ion current exists in rabbit sino-atrial node cells.
    • This current is likely mediated by P2-purinoreceptors and is permeable to various monovalent cations.
    • The identified current may contribute to the chronotropic effects observed upon autonomic nerve stimulation.