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Helium-neon laser irradiation is not a stressful treatment: a study on heat-shock protein (HSP70) level
I A Hashieh1, C Tardieu, J C Franquin
1Faculté d'Odontologie, Marseille, France.
Background And Objective:
Helium-neon (He-Ne) laser irradiation has been clinically used to reduce chemotherapy-induced mucositis. This work was designed to find out if this treatment is stressful at the cellular level by studying its effects on the level of the stress-inducible heat shock proteins.
Study Design/Materials And Methods:
Human desmodontal and mouse L929 fibroblasts were irradiated using a 60 mW laser by a single application of 1.5 and 3J/cm2 in continuous mode. Heat shock protein level was studied by gel electrophoresis and Western blotting using monoclonal antibodies.
Results:
He-Ne treatment does not induce heat shock protein synthesis in human desmodontal nor in mouse fibroblasts at the energy densities used in this study.
Conclusions:
These results indicate that the treatment is not stressful at the cellular level.