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Related Experiment Videos

Microtubule release from the centrosome

T J Keating1, J G Peloquin, V I Rodionov

  • 1Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA. tkeating@facstaff.wisc.edu

Proceedings of the National Academy of Sciences of the United States of America
|May 13, 1997
PubMed
Summary

Noncentrosomal microtubules (MTs) originate from constitutive nucleation and release at the centrosome. This release mechanism explains MT organization in differentiated cells and contributes to polymer dynamics.

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Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics

Background:

  • Microtubules (MTs) are typically assumed to originate from the centrosome.
  • However, some cell types exhibit substantial populations of noncentrosomal MTs, whose origin remains unclear.

Purpose of the Study:

  • To investigate the origin of noncentrosomal microtubules (MTs) using kinetic analysis in vivo.
  • To elucidate the formation and dynamics of MTs independent of direct centrosomal attachment.

Main Methods:

  • Applied kinetic analysis of MT formation in vivo.
  • Utilized time-lapse fluorescence microscopy in cultured epithelial cells.
  • Performed laser-marking experiments to track individual MT behavior.

Main Results:

  • Demonstrated that noncentrosomal MTs arise from constitutive nucleation and release from the centrosome.

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  • Observed released MTs moving individually with plus ends leading, suggesting minus end-directed motor transport.
  • Showed that released MTs are dynamic, with dynamic plus ends and stable or shortening minus ends.

    • Conclusions:

    • Centrosomal release and transport contribute to the formation of noncentrosomal MT arrays in differentiated cells.
    • MT release provides additional sites for subunit loss, influencing overall polymer turnover.
    • The noncentrosomal MT population likely represents a steady-state balance of centrosomal nucleation, release, and dynamics.