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Plasminogen activator system in osteoclasts

J N Yang1, E H Allan, G I Anderson

  • 1University of Southern California, School of Dentistry, Los Angeles 90089-0641, U.S.A.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|May 1, 1997
PubMed
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Osteoclasts express key genes of the plasminogen activator (PA) system, including urokinase receptor (uPAR) variants. This suggests a potential functional role for the PA system in osteoclast biology.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The plasminogen activator (PA) system plays crucial roles in extracellular matrix remodeling.
  • Osteoclasts are critical cells involved in bone resorption and remodeling.

Purpose of the Study:

  • To investigate the expression of plasminogen activator (PA) system genes in mouse osteoclasts.
  • To identify potential functional roles of the PA system in osteoclast activity.

Main Methods:

  • Microisolation of mouse osteoclasts.
  • Reverse transcribed polymerase chain reaction (RT-PCR) using gene-specific primers.
  • In situ hybridization with a urokinase receptor isoform 1 (uPAR1) probe.

Main Results:

Related Experiment Videos

  • Detected RNAs for tissue-type PA, urokinase-type PA, PA inhibitor-1, PA inhibitor-2, protease nexin, and uPAR1 in mouse osteoclasts.
  • Confirmed uPAR RNA expression in osteoclasts via in situ hybridization.
  • Identified a novel unspliced uPAR RNA variant alongside uPAR1 in osteoclasts, also found in osteoblasts, kidney, and muscle.

Conclusions:

  • Osteoclasts express a comprehensive set of PA system components.
  • The presence of these components suggests a significant functional role for the PA system in osteoclasts.
  • A novel uPAR RNA variant is expressed in osteoclasts and other mouse tissues.