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Capillary electrophoresis in pharmaceutical analysis

L A Holland1, N P Chetwyn, M D Perkins

  • 1Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66047, USA.

Pharmaceutical Research
|April 1, 1997
PubMed
Summary

Capillary electrophoresis (CE) offers powerful pharmaceutical analysis through various detection modes and sample preparation techniques. This review details advanced CE applications, including affinity, chiral, and chip-based methods for drug analysis.

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Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Pharmaceutical Analysis

Background:

  • Capillary electrophoresis (CE) is a versatile separation technique.
  • Pharmaceutical compound analysis requires precise and efficient separation methods.

Purpose of the Study:

  • To review common detection modes used in CE for pharmaceutical analysis.
  • To discuss sample preparation and preconcentration techniques for CE.
  • To cover advanced CE applications in pharmaceutical analysis.

Main Methods:

  • Review of UV absorption, fluorescence, electrochemical, and mass spectrometry detection in CE.
  • Description of sample preparation and preconcentration methods (hydrophobic retention, affinity, stacking, isotachophoresis).
  • Detailed examination of affinity CE, chiral CE, capillary gel electrophoresis, capillary electrochromatography, and CE on a chip.

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Main Results:

  • CE coupled with various detectors provides effective pharmaceutical analysis.
  • Advanced sample preparation enhances CE sensitivity and scope.
  • Specialized CE techniques like affinity and chiral CE are crucial for complex pharmaceutical analyses.

Conclusions:

  • Capillary electrophoresis is a highly adaptable technique for pharmaceutical analysis.
  • Recent advancements significantly expand the utility of CE in drug discovery and quality control.
  • Further development in miniaturized and specialized CE formats promises future innovations.