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Crystallization and preliminary X-ray analysis of Escherichia coli peptidyl-tRNA hydrolase
E Schmitt1, M Fromant, P Plateau
1Laboratoire de Biochimie, Unité de Recherche Associée 1970 du Centre National de la Recherche Scientifique, Palaiseau, France.
Proteins
|May 1, 1997
Abstract:
Peptidyl-tRNA hydrolase from Escherichia coli, a monomer of 21 kDa, was overexpressed from its cloned gene pth and crystallized by using polyethylene glycol as precipitant. The crystals are orthorhombic and have unit cell parameters a = 47.24 A, b = 63.59 A, and c = 62.57 A. They belong to space group P2(1)2(1)2(1) and diffract to better than 1.2 A resolution. The structure is being solved by multiple isomorphous replacement.