Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antiphospholipid antibodies: basic immunology and assays

D R Schultz1

  • 1Department of Medicine, University of Miami School of Medicine, FL 33101, USA.

Seminars in Arthritis and Rheumatism
|April 1, 1997
PubMed
Summary

Antiphospholipid antibodies (a-PL Abs), including anticardiolipin antibodies (a-CL Abs) and lupus anticoagulants (LAC), are crucial in autoimmune disorders. Research details their biochemical properties, cofactor interactions like beta 2-glycoprotein I (β2GPI), and diagnostic assays for antiphospholipid syndrome.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of an integrative cross-omics approach for conceptual adverse outcome pathway network construction.

Environment international·2026
Same author

Use of the phosphorus detector as a confirmatory tool for measuring residues of shell chemical's triazine herbicide.

Bulletin of environmental contamination and toxicology·2013
Same author

A non-statistical atomic model for beam emission and motional Stark effect diagnostics in fusion plasmas.

The Review of scientific instruments·2012
Same author

Kidney transplantation outcomes in African-, Hispanic- and Caucasian-Americans with lupus.

Lupus·2011
Same author

Creating and manipulating vortices in atomic wave functions with short electric field pulses.

Physical review letters·2011
Same author

Review: Lupus nephritis: pathologic features, epidemiology and a guide to therapeutic decisions.

Lupus·2010

Area of Science:

  • Biochemistry and Immunology
  • Focus on antiphospholipid antibodies (a-PL Abs) and their role in autoimmune diseases.

Background:

  • Antiphospholipid antibodies (a-PL Abs), including anticardiolipin antibodies (a-CL Abs) and lupus anticoagulants (LAC), are key players in autoimmune disorders.
  • Historical and current research on the biochemical and immunological aspects of a-PL Abs, phospholipids, and associated cofactors.

Purpose of the Study:

  • To review the biochemical and immunologic research on antiphospholipid antibodies (a-PL Abs).
  • To discuss the role of beta 2-glycoprotein I (β2GPI) as a cofactor.
  • To detail the characterization of lupus anticoagulants (LAC) and their diagnostic assays.

Main Methods:

  • Biochemical and structural analysis of phospholipids and antibodies.
  • Immunological assays for detecting a-PL Abs.

Related Experiment Videos

  • Characterization of cofactor interactions, particularly with beta 2-glycoprotein I (β2GPI).

    • Main Results:

    • Anticardiolipin antibodies (a-CL Abs) can cross-react with other anionic phospholipids.
    • Lupus anticoagulants (LAC) are heterogeneous and require specific assays for detection.
    • Beta 2-glycoprotein I (β2GPI) is a critical cofactor for autoimmune a-CL Abs binding to cardiolipin.

    Conclusions:

    • Antiphospholipid antibodies (a-PL Abs) and their associated cofactors are vital for understanding antiphospholipid syndrome.
    • Advanced diagnostic assays like ELISA have improved the detection and understanding of a-PL Abs.
    • Further research into LAC heterogeneity and cofactor interactions is essential for accurate diagnosis and management.