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Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra
C J Hackbarth1, I Unsal, H F Chambers
1Department of Medicine, University of California, San Francisco 94143, USA. corinne@itsa.ucsf.edu
Antimicrobial Agents and Chemotherapy
|May 1, 1997
Abstract:
A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.