Absolute identification of Hb D-Los Angeles (beta121 Glu replaced by Gln)

This paper describes a method which permits the absolute identification of Hb D-Los Angeles without the need for N-terminal or amino acid analysis on peptide beta DT-13. The procedure involves the formation of the 5-oxo-2-pyrrolidinecarboxyl derivative of the N-terminal glutamine residue ot betaDT-13. This method, together with the use of cadmium acetate/ninhydrin staining, allows the identification of Hb D-Los Angeles, and other possible mutations of beta121, by peptide mapping.