Involvement of GABAB receptors in the antinociception induced by baclofen in the formalin test

1. The effect of GABA receptor antagonists on baclofen-induced antinociception was examined in rats using the formalin test. Intraperitoneal (IP) administration of different doses of baclofen (2.5-10 mg kg-1) to rats induced antinociception in both phases of the test. 2. The response was dose-dependent and the maximum response was observed with 10 mg kg-1 of the drug. Intracerebroventricular (ICV) injection of baclofen (0.5-20 micrograms/rat) also induced dose-dependent antinociception in the second phase of the formalin test. 3. The GABAB antagonist, phaclofen (1 mg kg-1, IP) but not the GABAA antagonists picrotoxin (1 mg kg-1, IP) and bicuculline (1.5 mg kg-1, IP), decreased the antinociception induced by both ICV and IP administration of baclofen. 4. It is concluded that baclofen antinociception in the formalin test is mediated through GABAB receptor activation.