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Related Experiment Videos

Rapid antigen testing for group A Streptococcus by DNA probe

J S Heelan1, S Wilbur, G Depetris

  • 1Department of Pathology, Memorial Hospital of Rhode Island, Pawtucket, USA.

Diagnostic Microbiology and Infectious Disease
|February 1, 1996
PubMed
Summary

The Gen-Probe Group A Streptococcus Direct test (GASD) accurately detects Group A Streptococcus (GAS) from throat swabs. This rapid nucleic acid probe assay offers high sensitivity and specificity for diagnosing streptococcal pharyngitis.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Molecular Biology

Background:

  • Group A Streptococcus (GAS) is a common cause of pharyngitis.
  • Accurate and rapid diagnostic methods are crucial for effective treatment.
  • Nucleic acid probe assays offer potential for improved diagnostic speed and accuracy.

Purpose of the Study:

  • To evaluate the performance of the Gen-Probe Group A Streptococcus Direct test (GASD) for detecting GAS from throat swabs.
  • To compare the GASD assay results with traditional culture methods.
  • To determine the sensitivity, specificity, and predictive values of the GASD assay.

Main Methods:

  • A nucleic acid probe assay (GASD) utilizing an acridium ester-labeled DNA probe targeting Streptococcus pyogenes rRNA was employed.

Related Experiment Videos

  • 318 symptomatic outpatient throat swabs were tested.
  • Culture on sheep blood agar served as the gold standard, with swabs stored at 4°C for up to 72 hours before testing.

    • Main Results:

    • The GASD assay demonstrated high diagnostic accuracy.
    • Sensitivity was 91.4%, specificity was 97%, positive predictive value was 91.4%, and negative predictive value was 97% after discrepancy testing.
    • The test is suitable for batch screening.

    Conclusions:

    • The Gen-Probe Group A Streptococcus Direct test (GASD) is a rapid and user-friendly method for the batch screening of streptococcal pharyngitis.
    • The assay provides reliable results comparable to traditional culture methods.
    • GASD offers a valuable tool for clinical laboratories managing suspected cases of GAS infections.