[Amine oxidase from the bacterial strain Methanosarcina barkeri 27]

A new source of the enzyme amine oxidase (AO), namely bacteria of Methanosarcina barkery 27 strain, was discovered. The method AO isolation and purification from the bacteria was developed and the enzyme substrate and inhibitory specificity was studied. Contrary to some other microbial amine oxidases the AO investigated was shown to determine benzylamine and serotonin along with tyramine, all these substrates being deaminated at the equal rate. By contrast to other microbial AO the AO discovered was unable to deaminate histamine. The AO properties have been compared with those of mitochondrial monoamine oxidase from pig liver.