Effect of epirubicin on 3H-thymidine labelling index in cultured L-strain cells

The effect of epirubicin (EPI) on 3H-thymidine labelling index (3H-TdR LI) of L-strain cells in culture was investigated. EPI concentrations of 0.001 microg/ml, 0.01 microg/ml and 0.1 microg/ml were applied to the cells for 4, 8, 16 and 32 hours. Following the treatment with EPI, labelling process in 3H-TdR medium continued for 0, 2, 4, 8, 16, 20, 22, 25 and 30 hours. The results showed that EPI diminished labelling index of L-strain cells depends on time and applied concentrations. When compared to control this decrease was found statistically significant (p<0.001) in each group.