Related Experiment Videos
Microplate assay for measurement of histamine release from mast cells
1Oklahoma State University, College Osteopathic Medicine, Tulsa, USA. price@vms.ocom.okstate.edu
Biotechniques
|May 1, 1997
Abstract:
A microplate-format assay for measurement of histamine release by activated rat peritoneal mast cells, with accompanying microplate-format spectrofluorometric assay of histamine, is demonstrated. Reproducibility and linearity of the histamine assay are comparable to the historical large format assay. Transfer of samples in the 96-well format, from histamine release through spectrofluorometric assay, greatly expedites handling and provides for better independence of each replicate. A sample experiment is included to show a typical size experiment easily done in this format.