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Related Experiment Videos

dad-1, A putative programmed cell death suppressor gene in rice

Y Tanaka1, T Makishima, M Sasabe

  • 1Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Japan.

Plant & Cell Physiology
|March 1, 1997
PubMed
Summary

Rice plants possess a gene similar to the human dad-1 gene. This plant gene effectively prevents programmed cell death in animal cells, indicating a conserved function in cell survival across species.

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Area of Science:

  • Molecular Biology
  • Plant Science
  • Cell Biology

Background:

  • The dad-1 gene is crucial for preventing programmed cell death in animals.
  • Understanding the evolutionary conservation of dad-1 function can provide insights into fundamental cellular processes.

Purpose of the Study:

  • To investigate the presence and function of a dad-1 homolog in rice (Oryza sativa).
  • To determine if the rice dad-1 homolog can functionally complement animal dad-1 mutants.

Main Methods:

  • Isolation and characterization of the rice dad-1 cDNA homolog.
  • Expression of the rice dad-1 homolog in temperature-sensitive hamster cell mutants lacking functional dad-1.
  • Assessment of cell viability and apoptosis in rescued cell lines.

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Main Results:

  • A rice cDNA homolog of human dad-1 was successfully isolated.
  • The amino acid sequence showed significant conservation between rice and animal dad-1 proteins.
  • The rice dad-1 homolog rescued temperature-sensitive hamster cell mutants from apoptotic cell death.

Conclusions:

  • The rice dad-1 homolog possesses a conserved function as a suppressor of programmed cell death.
  • This finding suggests a fundamental role for dad-1 in cell survival pathways across diverse eukaryotic organisms.