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Human chromosome aberrations induced in vitro by Paracoccidioides brasiliensis glycoproteic component (GP 43)
D V Freire-Maia1, M A Balarin, M J Mendes-Giannini
1Department of Genetics, UNESP, Botucatu-SP, Brazil.
Abstract:
The in vitro cytogenetic effects of the 43-kDa molecular mass exocellular glycoproteic component (GP 43) from Paracoccidioides brasiliensis were studied in cultures from human lymphocytes. The sample included 10 healthy, white, non-smoking, non-related males (mean age of 31.3 +/- 8.2 years). Besides the control, three concentrations of GP 43 (0.125, 1.25 and 5 micrograms/ml) were used. In each group, around 1000 cells were examined in search of chromosome aberrations, and 30000 metaphases were analysed for the determination of the Mitotic Index. The authors conclude that GP 43 most probably causes inhibition of the cell cycle and aneugenic and clastogenic effects.
Insights
The Paracoccidioides brasiliensis glycoprotein 43 (GP 43) component likely inhibits cell cycle progression. This study found GP 43 induces both aneugenic and clastogenic effects in human lymphocytes.
Area of Science:
- Medical Mycology
- Cytogenetics
- Molecular Biology
Background:
- Paracoccidioides brasiliensis is a fungus causing paracoccidioidomycosis.
- Exocellular glycoproteins, like GP 43, are key virulence factors.
- Understanding fungal component effects on host cells is crucial.
Purpose of the Study:
- To investigate the in vitro cytogenetic effects of GP 43 from P. brasiliensis.
- To determine if GP 43 induces chromosome aberrations or affects cell division.
- To assess the genotoxic potential of GP 43.
Main Methods:
- Human lymphocyte cultures were exposed to varying concentrations of GP 43.
- Cells were analyzed for chromosome aberrations.
- The Mitotic Index was determined to assess cell proliferation.
Main Results:
- GP 43 exposure led to observable chromosome aberrations.
- A significant impact on the Mitotic Index was noted, suggesting cell cycle inhibition.
- Both aneugenic (chromosome loss/gain) and clastogenic (chromosome breakage) effects were indicated.
Conclusions:
- GP 43 most probably causes inhibition of the cell cycle.
- The study concludes GP 43 exhibits aneugenic and clastogenic effects.
- GP 43 demonstrates genotoxic potential in human lymphocytes.
