Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The translational stop signal: codon with a context, or extended factor recognition element?

W P Tate1, E S Poole, M E Dalphin

  • 1Department of Biochemistry, University of Otago, Dunedin, New Zealand.

Biochimie
|January 1, 1996
PubMed
Summary

The translational stop signal is more than just a stop codon; it is an extended sequence recognized by release factors. This sequence, particularly the base following the stop codon, dictates termination efficiency in vivo.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changes in DNA methylation profiles of myalgic encephalomyelitis/chronic fatigue syndrome patients reflect systemic dysfunctions.

Clinical epigenetics·2020
Same author

Demonstration of non-inferiority of a novel combination intramammary antimicrobial in the treatment of clinical mastitis.

New Zealand veterinary journal·2016
Same author

After the genome sequencing of duckweed - how to proceed with research on the fastest growing angiosperm?

Plant biology (Stuttgart, Germany)·2015
Same author

Evaluation of phylogenetic relationships in Lemnaceae using nuclear ribosomal data.

Plant biology (Stuttgart, Germany)·2014
Same author

Development of a comprehensive set of P2 receptor pharmacological research compounds.

Purinergic signalling·2011
Same author

Allozyme diversity in endemic flowering plant species of the Juan Fernandez Archipelago, Chile: ecological and historical factors with implications for conservation.

American journal of botany·2011

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Previous studies on translational stop codon readthrough suggested context-dependent termination.
  • Existing research often failed to differentiate between stop codon suppression and termination effects.

Purpose of the Study:

  • To determine if the translational stop signal is a codon with context or an extended factor recognition element.
  • To investigate the molecular signature of translational termination signals.

Main Methods:

  • Analysis of a large database of natural termination sites across 148 organisms (~40,000 sequences).
  • In vivo studies in Escherichia coli, including overexpression of release factor 3.
  • Site-directed cross-linking experiments with bacterial release factor-2.

Related Experiment Videos

Main Results:

  • A significant bias in bases surrounding stop codons was observed across all organisms, with a strong preference at the +4 position.
  • The base at the +4 position influences the efficiency of the stop signal in vivo.
  • Bases from +1 to +6, but not beyond, directly contact bacterial release factor-2.

Conclusions:

  • The translational stop signal is an extended factor recognition sequence, not just a triplet codon influenced by context.
  • This extended sequence includes a core element crucial for termination.
  • The findings provide a more comprehensive understanding of translational termination mechanisms.