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A general method for DNA polymorphism identification in genetic assessment and molecular diagnosis
K Beyer1, J I Lao, X A Alvarez
1EuroEspes Biomedical Research Center, Department of Clinical and Molecular Genetics, Molecular Neuropharmacology and Neurogeriatrics, La Coruña, Spain.
Abstract:
A general methodology for the analysis of human diseases at the nucleic acid level has been described. This method consists of a semiautomated, nontoxic analytic procedure, staining DNA bands with silver nitrate instead of ethidium bromide after DNA amplification by PCR. This technique provides a sensitive assay with enhanced accuracy and offers the possibility of carrying out the molecular analysis of several polymorphisms in a single day simplifying molecular genetics with diagnostic purposes.