Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diversity within bacterial isolates hybridizing with Comamonas probe ppT

T T Koivula1, J Hantula

  • 1Department of Biosciences, University of Helsinki, Finland.

Journal of Basic Microbiology
|January 1, 1997
PubMed
Summary

Bacteria from activated sludge and the Baltic Sea, initially identified using a specific probe, were analyzed. Findings indicate these bacteria represent two distinct, previously unknown species, not *C. testosteroni*.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Report of Phytophthora ramorum and P. inflata in Ornamental Rhododendrons in Finland.

Plant disease·2019
Same author

First Observations of Mycosphaerella pini on Scots Pine in Finland.

Plant disease·2019
Same author

Characterization of small RNAs originating from mitoviruses infecting the conifer pathogen Fusarium circinatum.

Archives of virology·2018
Same author

Heterobasidion wood decay fungi host diverse and globally distributed viruses related to Helicobasidium mompa partitivirus V70.

Virus research·2014
Same author

Biogeographical patterns and determinants of invasion by forest pathogens in Europe.

The New phytologist·2012
Same author

Morphological measurements and ITS sequences show that the new alder rust in Europe is conspecific with Melampsoridium hiratsukanum in eastern Asia.

Mycologia·2009

Area of Science:

  • Microbiology
  • Bacterial Taxonomy
  • Environmental Microbiology

Background:

  • Bacterial identification relies on specific molecular probes and phenotypic characteristics.
  • Previous studies utilized rRNA-targeted probes to identify bacterial species.
  • The probe ppT was designed to identify *C. testosteroni*.

Purpose of the Study:

  • To investigate the diversity of bacteria recognized by the ppT probe in activated sludge and Baltic Sea samples.
  • To determine if these isolates belong to the species *C. testosteroni*.
  • To characterize novel bacterial species from these environments.

Main Methods:

  • Isolation of bacteria from activated sludge and Baltic Sea environments.
  • Partial sequencing of 16S ribosomal RNA (rRNA) genes for phylogenetic analysis.

Related Experiment Videos

  • Phage and bacteriocin sensitivity assays.
  • Whole-cell protein pattern analysis.

    • Main Results:

    • Activated sludge and Baltic Sea bacterial isolates formed distinct phylogenetic groups based on 16S rRNA gene sequences.
    • These groups were separate from *C. testosteroni*.
    • Phage/bacteriocin sensitivity and protein profiles corroborated the distinct groupings, with some intragroup variation observed.

    Conclusions:

    • The bacteria studied from activated sludge and the Baltic Sea are not *C. testosteroni*.
    • These isolates likely represent two novel bacterial species.
    • The findings contribute to understanding microbial diversity in aquatic and wastewater environments.