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Tenascin supports lymphocyte rolling

R A Clark1, H P Erickson, T A Springer

  • 1The Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.

The Journal of Cell Biology
|May 5, 1997
PubMed
Summary
This summary is machine-generated.

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Tenascin supports lymphocyte rolling on extracellular matrix under flow conditions, suggesting a novel mechanism for immune cell migration in lymphoid organs.

Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • Tenascin is an extracellular matrix protein found in immune tissues.
  • Its adhesive and anti-adhesive properties have been previously described in static assays.

Purpose of the Study:

  • To investigate the role of tenascin in lymphocyte adhesion and migration under flow conditions.
  • To identify the binding domain and mechanism of lymphocyte interaction with tenascin.

Main Methods:

  • Lymphocyte tethering and rolling assays under physiological flow conditions.
  • Biochemical treatments including enzymatic digestion (O-glycoprotease, glycosidases, proteinase K) and calcium dependency studies.
  • Antibody blocking and binding studies using recombinant tenascin proteins and specific domains.

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Main Results:

  • Tenascin supports the tethering and rolling of lymphocytes and lymphoblastic cell lines under flow.
  • Binding is calcium-dependent and involves the fibrinogen-like domain of tenascin.
  • Lymphocyte rolling on tenascin is smoother and involves more bonds compared to E-selectin, requiring higher tenascin density.

Conclusions:

  • Tenascin facilitates lymphocyte rolling via a novel receptor, distinct from known tenascin receptors.
  • This interaction likely contributes to lymphocyte migration through secondary lymphoid organs.
  • The findings suggest a new mechanism for immune cell trafficking mediated by extracellular matrix components.