Viability and metabolic activity of homozygous Brachyury (T) embryos

Some features of metabolic activity of homozygous mutants for the Brachyury (T) mutation were studied. The embryos incorporated tritum-labelled thymidine up to about the '32-36 somite' stage. The total amount of protein per embryo increased until the samge stage. T/T cells proved viable in vitro over the in utero lethal period. Several cell lines were established from +/+ and T/T embryonic cells.