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Related Experiment Videos

Two naturally occurring transposons indistinguishable from Tn7

P T Barth, N Datta

    Journal of General Microbiology
    |September 1, 1977
    PubMed
    Summary

    Transposons Tn71 and Tn72, conferring antibiotic resistance, are molecularly identical to the known Tn7 transposon. This suggests natural, widespread dissemination of the Tn7 transposon among bacterial plasmids.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Antibiotic resistance is a growing global health concern.
    • Plasmids are key vectors for the spread of antibiotic resistance genes.
    • Transposons facilitate the movement of genetic material, including antibiotic resistance genes, between plasmids.

    Purpose of the Study:

    • To characterize two novel transposons, Tn71 and Tn72, identified on plasmids.
    • To compare the genetic and molecular properties of Tn71 and Tn72 with the known Tn7 transposon.
    • To investigate the potential for natural spread of the Tn7 transposon.

    Main Methods:

    • Plasmid isolation and characterization.
    • Restriction enzyme digestion analysis (EcoRI, HindIII, BamHI) to compare DNA fragment patterns.
    • Molecular mass determination of transposons.

    Main Results:

    • Two plasmids carrying trimethoprim and streptomycin resistance genes were identified.
    • The transposons within these plasmids, designated Tn71 and Tn72, were found to be indistinguishable from Tn7.
    • Comparisons of molecular mass and restriction enzyme digestion sites confirmed the identity with Tn7.

    Conclusions:

    • The findings indicate that Tn71 and Tn72 are identical to the Tn7 transposon.
    • The data strongly support the natural and widespread dissemination of the Tn7 transposon among plasmids.
    • This highlights a significant mechanism for the spread of antibiotic resistance.

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