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Related Experiment Videos

Rok1p is a putative RNA helicase required for rRNA processing

J Venema1, C Bousquet-Antonelli, J P Gelugne

  • 1European Molecular Biology Laboratory, Heidelberg, Germany. venema@chem.vu.nl

Molecular and Cellular Biology
|June 1, 1997
PubMed
Summary

The ROK1 gene is essential for ribosome synthesis in yeast, particularly for early pre-ribosomal RNA (rRNA) processing. Rok1p, an RNA helicase, is crucial for the function of small nucleolar ribonucleoprotein particles (snoRNPs) in this vital cellular process.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Ribosome synthesis is a fundamental cellular process requiring numerous transacting factors, including small nucleolar ribonucleoprotein particles (snoRNPs).
  • Specific snoRNAs and snoRNP proteins, like snR10 and Gar1p, are known to be involved in early pre-ribosomal RNA (rRNA) processing steps crucial for 18S rRNA synthesis.

Purpose of the Study:

  • To identify novel genetic factors involved in early pre-rRNA processing and ribosome biogenesis.
  • To elucidate the role of the DEAD-box RNA helicase Rok1p in snoRNP function and pre-rRNA maturation.

Main Methods:

  • Genetic screening for synthetic lethality with mutations affecting snoRNP function (snR10 deletion or temperature-sensitive gar1 allele).
  • Gene essentiality and phenotypic analysis (growth impairment, pre-rRNA processing defects) upon gene depletion.

Related Experiment Videos

  • Subcellular localization studies using indirect immunofluorescence.

    • Main Results:

    • The ROK1 gene, encoding an ATP-dependent DEAD-box RNA helicase, was identified through genetic screens.
    • ROK1 is essential for yeast viability, and its depletion inhibits pre-rRNA processing at sites A0, A1, and A2, blocking 18S rRNA synthesis.
    • Rok1p localizes predominantly to the nucleolus, the site of ribosome biogenesis.

    Conclusions:

    • Rok1p is a novel essential factor required for early pre-rRNA cleavage events.
    • These findings suggest Rok1p functions within or in conjunction with snoRNP complexes to facilitate pre-rRNA processing.
    • Rok1p plays a critical role in the maturation of 18S rRNA during ribosome synthesis.