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Related Experiment Videos

The growth hormone-dependent decrease in hepatic fatty acid synthase mRNA is the result of a decrease in gene

S S Donkin1, A D McNall, B S Swencki

  • 1Department of Dairy and Animal Science, The Pennsylvania State University, University Park 16802, USA.

Journal of Molecular Endocrinology
|April 1, 1996
PubMed
Summary

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Porcine growth hormone treatment significantly boosted growth rates in rats. This hormone also reduced fatty acid synthase (FAS) mRNA by decreasing gene transcription, indicating specific metabolic regulation.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Background:

  • Growth hormone (GH) plays a crucial role in mammalian growth and metabolism.
  • Fatty acid synthase (FAS) is a key enzyme in lipogenesis, regulated by hormones like insulin.
  • Understanding GH's impact on FAS is vital for metabolic research.

Purpose of the Study:

  • To investigate the chronic effects of porcine growth hormone (pGH) on fatty acid synthase (FAS) gene expression in rats.
  • To determine if pGH influences FAS mRNA abundance and gene transcription rates.
  • To assess the specificity of pGH effects on insulin-regulated genes.

Main Methods:

  • Rats were administered porcine growth hormone chronically.
  • Quantitative analysis of fatty acid synthase (FAS) mRNA levels was performed.

Related Experiment Videos

  • Gene transcription rates for FAS and phosphoenolpyruvate carboxykinase (PEPCK) were measured.

    • Main Results:

    • Porcine growth hormone administration increased rat growth rate by 27%.
    • FAS mRNA abundance decreased by 55% due to an 80% reduction in FAS gene transcription.
    • pGH did not alter mRNA levels or transcription of the insulin-regulated gene PEPCK.

    Conclusions:

    • Chronic porcine growth hormone treatment reduces fatty acid synthase (FAS) mRNA levels primarily by decreasing gene transcription.
    • The effects of growth hormone on FAS gene expression are specific and not mediated by general alterations in insulin-responsive gene regulation in the liver.