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[Obtaining donor corneas without eyeball removal]

A Künster1, U Eckardt, C Eckardt

  • 1Augenklinik, Städtische Kliniken, Frankfurt am Main/Höchst.

Der Ophthalmologe : Zeitschrift Der Deutschen Ophthalmologischen Gesellschaft
|February 1, 1997
PubMed
Summary

Directly obtained 7-mm corneal buttons stored in Optisol match the quality of standard corneal transplants. This method offers a viable alternative for donor tissue preparation, potentially improving corneal transplantation outcomes.

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Area of Science:

  • Ophthalmology
  • Tissue Engineering
  • Materials Science

Context:

  • Corneal transplantation relies on high-quality donor tissue.
  • Current methods involve preparing larger specimens and then excising the desired corneal button.
  • The preservation and preparation methods impact graft integrity and surgical success.

Purpose:

  • To compare the quality of 7-mm corneal buttons obtained directly from donor eyes with those trephined from larger corneoscleral specimens.
  • To evaluate the effects of Optisol storage on corneal thickness, endothelial cell viability, and structural integrity.

Summary:

  • Porcine corneal specimens (7-mm and 16-mm) were stored in Optisol for 3 and 7 days. Ultrasonic pachymetry showed initial thickness reduction, recovering to 91% by day 8. Light and scanning electron microscopy revealed homogeneous endothelial layers, minimal cell death, and smooth cutting edges in both groups.

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  • Human 7-mm corneal buttons, some obtained directly and others trephined post-immersion, showed no significant differences in quality, including endothelial integrity and cutting edge smoothness.
  • The study indicates that preparing 7-mm corneal buttons in situ and storing them in Optisol yields results comparable to conventional methods.

    • Impact:

    • This research suggests a potential optimization in donor corneal tissue preparation, possibly leading to improved efficiency and graft quality in corneal transplantation.
    • The findings support the viability of direct procurement and storage of 7-mm corneal buttons, warranting further clinical investigation.
    • This could influence future protocols for donor eye banking and corneal graft preparation, ultimately benefiting patients awaiting transplantation.