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Related Experiment Videos

Macromolecular transport in malaria--does the duct exist?

A R Hibbs1, D J Stenzel, A Saul

  • 1Queensland Institute of Medical Research, Royal Brisbane Hospital, Brisbane/Australia.

European Journal of Cell Biology
|February 1, 1997
PubMed
Summary

This study challenges the existence of a 'duct' in malaria-infected erythrocytes, finding that observed molecular uptake is due to dye release, not sphere transport. This suggests no open channel exists for macromolecule transport in malaria.

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Area of Science:

  • Malariology
  • Cell Biology
  • Parasitology

Background:

  • The proposed 'duct' in malaria-infected erythrocytes influences understanding of macromolecular transport and parasite immunobiology.
  • Evidence for this 'duct' relied on experiments using fluorescent latex spheres, suggesting a direct connection between the parasite and its environment.

Purpose of the Study:

  • To investigate the nature of macromolecular transport in malaria-infected erythrocytes.
  • To re-evaluate the evidence for a parasitophorous 'duct' and its role in molecular uptake.

Main Methods:

  • Replication of experiments using fluorescent latex spheres of varying sizes (down to 14 nm).
  • Analysis of fluorescent labeling to distinguish between sphere uptake and artifactual dye release.

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Main Results:

  • Fluorescent labeling was attributed to dye release from latex spheres, not the uptake of the spheres themselves.
  • Malaria-infected erythrocytes demonstrated an inability to uptake latex beads as small as 14 nm in diameter.

Conclusions:

  • The existence of an open 'duct' connecting the malaria parasite to the erythrocyte exterior is refuted.
  • Macromolecular transport in malaria-infected erythrocytes is not facilitated by such a channel, necessitating a re-evaluation of existing models.