Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

beta-Lactamases are absent from Archaea (archaebacteria)

H H Martin1, H König

  • 1Fachbereich 10-Biologie-Technische Hochschule Darmstadt, Germany.

Microbial Drug Resistance (Larchmont, N.Y.)
|July 1, 1996
PubMed
Summary

Beta-lactamase enzymes, which degrade beta-lactam antibiotics, are common in bacteria. This study found no evidence of these enzymes in Archaea or Eucarya, suggesting their restriction to bacteria.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dialectical behavior therapy (DBT) in an assertive community treatment structure (ACT): testing integrated care borderline (ICB) in a randomized controlled trial (RECOVER).

Borderline personality disorder and emotion dysregulation·2024
Same author

An implementation manual for an interprofessional enhanced recovery after surgery protocol in cardiac surgery following international established frameworks.

Frontiers in cardiovascular medicine·2024
Same author

Three by three weeks of robot-enhanced repetitive gait therapy within a global rehabilitation plan improves gross motor development in children with cerebral palsy - a retrospective cohort study.

European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society·2019
Same author

The excess costs of depression: a systematic review and meta-analysis.

Epidemiology and psychiatric sciences·2019
Same author

[Developmental neurology - networked medicine and new perspectives].

Der Nervenarzt·2017
Same author

[Migraine in children and adolescents-brain and muscle? : Another example of why children are not small adults].

Der Nervenarzt·2017

Area of Science:

  • Microbiology
  • Biochemistry

Background:

  • Beta-lactamases are enzymes crucial for bacterial resistance to beta-lactam antibiotics.
  • These enzymes are involved in bacterial cell wall metabolism.
  • The presence of beta-lactamases in Archaea, a distinct prokaryotic domain, remains unexplored.

Purpose of the Study:

  • To investigate the presence or absence of beta-lactamase activity in various archaeal species.
  • To determine if beta-lactamases are exclusive to the bacterial domain.

Main Methods:

  • Tested diverse archaeal organisms (methanogenic and halophilic) with different cell wall compositions.
  • Utilized nitrocefin, a chromogenic substrate, to detect beta-lactamase activity.
  • Included representative eukaryotic microorganisms (algae, yeasts, protozoa) for comparison.

Main Results:

  • No detectable beta-lactamase activity was observed in any of the tested archaeal samples.
  • Eukaryotic microorganisms also showed no beta-lactamase activity.
  • The findings indicate a lack of these enzymes in the archaeal and eukaryotic domains.

Conclusions:

  • Beta-lactamase activity appears to be absent in Archaea and Eucarya.
  • This study supports the hypothesis that beta-lactamases are exclusively found in Bacteria.

Related Experiment Videos