Factors influencing the preimmune antibody repertoire
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Updated: Nov 1, 2026

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
1Laboratory of Immunobiology, Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
Abstract:
Preimmune antibody repertoire is not a statistical representation of all germ-line VH, D, JH and VL, JL segments encoding heavy and light chains, respectively. The antibody repertoire is biased towards a fraction of VH and VL gene segments in fetal/neonatal as well as adult life. The repertoire bias starts at the surface Ig-negative pre-B cells and persists throughout B-cell ontogeny. Antigen-independent processes like preferential rearrangements of VH, D and JH segments and pairing of the heavy and surrogate light chain operate at the surface Ig-negative pre-B cell stage. Subsequently B cells may be subject to self-antigen selection based on the specificity of their surface receptors.
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