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CREM: a transcriptional master switch during the spermatogenesis differentiation program

F Nantel1, P Sassone-Corsi

  • 1Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue Laurent Fries, BP 163, ILLKIRCH Cedex, C.U. de Strasbourg, France.

Frontiers in Bioscience : a Journal and Virtual Library
|September 1, 1996
PubMed
Summary

The CREM gene is essential for male fertility. Inactivating CREM halts sperm development and causes sterility in mice, highlighting its critical role in spermatogenesis.

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Area of Science:

  • Reproductive Biology
  • Molecular Genetics
  • Cellular Differentiation

Background:

  • Spermatogenesis is a complex process regulated by the hypothalamic-pituitary axis.
  • The CREM gene produces transcription factors influencing cAMP-mediated gene expression.
  • The CREMtau activator isoform is crucial for post-meiotic gene transcription in spermatogenesis.

Purpose of the Study:

  • To investigate the essential role of the CREM gene in spermatogenesis.
  • To determine the consequences of CREM gene inactivation on male fertility and germ cell development.

Main Methods:

  • Homologous recombination was used to generate CREM-inactivated mice.
  • Histological analysis of seminiferous tubules was performed.
  • Germ cell apoptosis levels were quantified.

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Main Results:

  • Homozygous male mutant mice lacking CREM were sterile with no spermatozoa.
  • Spermatogenesis arrested at the first step of spermiogenesis in mutant mice.
  • CREM deficiency led to a lack of post-meiotic gene expression and a ten-fold increase in germ cell apoptosis.

Conclusions:

  • The CREM gene plays an essential role in spermatogenesis.
  • CREM is required for post-meiotic gene expression and germ cell survival.
  • CREM inactivation in mice models aspects of male infertility.