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Related Experiment Videos

Intron open reading frames as mobile elements and evolution of a group I intron

C H Sellem1, L Belcour

  • 1Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, France. sellem@cgm.cnrs-gif.fr

Molecular Biology and Evolution
|May 1, 1997
PubMed
Summary

Mobile Group I introns, specifically the mitochondrial nad1-i4-orf1 in Podospora, demonstrate efficient mobility via open reading frame (ORF) transfer. This mobility is linked to intron evolution and splicing variations.

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Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Mycology

Background:

  • Group I introns are hypothesized to gain mobility through acquired open reading frames (ORFs) encoding DNA endonucleases.
  • Intron ORFs are thought to act as autonomously mobile genetic elements, supported by indirect evidence.

Purpose of the Study:

  • To experimentally demonstrate the mobility of intron ORFs through direct transfer.
  • To investigate the evolutionary history and splicing mechanisms of the mitochondrial nad1-i4 intron in Podospora.

Main Methods:

  • Conducted ORF transfer experiments between Podospora strains to assess intron mobility.
  • Utilized Polymerase Chain Reaction (PCR) to analyze intron splicing events and genomic organization.

Main Results:

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  • Demonstrated efficient mobility of the mitochondrial nad1-i4-orf1 via transfer between Podospora strains.
  • Observed bidirectional co-conversion during ORF homing, not always involving the entire intron sequence.
  • Identified a 5'-alternative splice site in a biorfic intron, likely a relic of its monoorfic ancestor.

Conclusions:

  • Intron ORF acquisition represents a recent evolutionary step for the nad1-i4 intron.
  • Proposed a sequential evolution model for the nad1 locus, encompassing no intron, intron-only, monoorfic, and biorfic states across Pyrenomycete species.