Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Induction of CD8+ CTL recognizing mycobacterial peptides

H M Vordermeier1, X Zhu, D P Harris

  • 1MRC Clinical Sciences Centre, Tuberculosis & Related Infections Unit, Hammersmith Hospital, London, UK.

Scandinavian Journal of Immunology
|May 1, 1997
PubMed
Summary

Researchers identified eight new CD8+ T cell epitopes from Mycobacterium tuberculosis proteins. These findings are crucial for understanding tuberculosis immunity and developing new vaccines against this infectious disease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vaccination of calves with Mycobacterium bovis Bacillus Calmette-Guerin reduces the frequency and severity of lesions of bovine tuberculosis under a natural transmission setting in Ethiopia.

Transboundary and emerging diseases·2017
Same author

Differential Cell Composition and Cytokine Expression Within Lymph Node Granulomas from BCG-Vaccinated and Non-vaccinated Cattle Experimentally Infected with Mycobacterium bovis.

Transboundary and emerging diseases·2016
Same author

Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting.

Veterinary immunology and immunopathology·2014
Same author

The consequences of vaccination with the Johne's disease vaccine, Gudair, on diagnosis of bovine tuberculosis.

The Veterinary record·2013
Same author

Upregulation of IL-17A, CXCL9 and CXCL10 in early-stage granulomas induced by Mycobacterium bovis in cattle.

Transboundary and emerging diseases·2012
Same author

Duration of immunity against Mycobacterium bovis following neonatal vaccination with bacillus Calmette-Guérin Danish: significant protection against infection at 12, but not 24, months.

Clinical and vaccine immunology : CVI·2012

Area of Science:

  • Immunology
  • Microbiology
  • Infectious Diseases

Background:

  • Mycobacterium tuberculosis is a leading cause of infectious morbidity.
  • CD8+ T cells are critical for anti-tuberculosis immune responses.
  • Limited knowledge exists on mycobacterial antigens recognized by CD8+ T cells, hindering vaccine development.

Purpose of the Study:

  • To identify novel CD8+ T cell epitopes from Mycobacterium tuberculosis.
  • To characterize mycobacterial antigens recognized by CD8+ T cells.
  • To explore the potential of these epitopes in tuberculosis vaccine development.

Main Methods:

  • Identified allele-specific MHC class I binding motifs in six M. tuberculosis protein antigens.
  • Predicted epitopes were tested for MHC binding and ability to elicit peptide-specific CTL.

Related Experiment Videos

  • Spleen cells from peptide-immunized mice were used to identify novel epitopes.
  • CTL recognition of naturally processed epitopes on transfected cells was assessed.

    • Main Results:

    • Six prominent M. tuberculosis protein antigens were analyzed for MHC class I binding motifs.
    • Eight previously undescribed mycobacterial CTL epitopes were identified.
    • Identified CTLs recognized naturally processed epitopes presented on target cells.

    Conclusions:

    • The identified mycobacterial CTL epitopes are valuable for studying CD8+ T cell roles in tuberculosis.
    • These epitopes offer potential targets for future tuberculosis vaccine development.
    • Further research can elucidate the involvement of CD8+ T cells in M. tuberculosis infection and pathogenesis.