Qualitative and quantitative measurements of oligonucleotides in gene therapy: Part I. In vitro models

Part I of this review attempts to bring together all the methods of detection and determination of synthetic oligonucleotides used in in vitro, described in the literature over the past 14 years, in an effort by scientists to use these oligonucleotides as drugs in gene therapy. The in vitro models include cell-free and cell culture systems. Emphasis has been given to the techniques developed for quantification of the input oligonucleotides or their metabolites. The purpose of study, methods of processing, detection and determination techniques such as those based on fluorescence, radiolabeling, high-performance liquid chromatography, gel-electrophoresis and others have been presented.