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Related Experiment Videos

Adenosine A3 receptor expression and function in eosinophils

B A Walker1, M A Jacobson, D A Knight

  • 1University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, Canada.

American Journal of Respiratory Cell and Molecular Biology
|May 1, 1997
PubMed
Summary
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The A3 adenosine receptor is mainly found on eosinophils in human lungs, where it inhibits their movement. This suggests A3 receptor drugs could treat diseases like asthma.

Area of Science:

  • Immunology
  • Pulmonology
  • Pharmacology

Background:

  • The A3 adenosine receptor's location and function in humans are unclear.
  • Adenosine affects inflammatory cells in the lungs, worsening airway narrowing in disorders.
  • Hypothesis: A3 receptor gene expression is on inflammatory cells and increases with airway inflammation.

Purpose of the Study:

  • To determine the cellular localization and function of A3 adenosine receptors in human lung tissue.
  • To investigate the role of A3 receptors in airway inflammation.

Main Methods:

  • In situ hybridization on lung and airway tissues from healthy and inflamed subjects.
  • Quantification of A3 receptor transcripts in purified blood cells (eosinophils, neutrophils, mononuclear cells).

Related Experiment Videos

  • Assessment of A3 receptor agonist/antagonist effects on eosinophil chemotaxis.

    • Main Results:

    • A3 receptors were found on mesenchymal cells and eosinophils in airways and blood vessels, not mast cells.
    • A3 receptor transcripts were significantly higher in eosinophils compared to neutrophils and mononuclear cells.
    • Lung tissue from inflamed subjects showed greater A3 receptor transcript abundance than normal lung.
    • An A3 receptor agonist inhibited eosinophil chemotaxis, an effect blocked by an A3 antagonist.

    Conclusions:

    • A3 adenosine receptors are primarily located on human lung eosinophils.
    • These receptors inhibit eosinophil chemotaxis, suggesting a role in regulating inflammation.
    • Targeting A3 receptors may offer a therapeutic strategy for eosinophil-driven diseases like asthma and rhinitis.