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The ovine SOX2 gene: sequence, chromosomal localization and gonadal expression
E Payen1, E Pailhoux, L Gianquinto
1Laboratoire de Biologie Cellulaire et Moléculaire, INRA, Batiment des Biotechnologies, Jouy en Josas, France.
Gene
|April 11, 1997
Summary
Researchers identified the ovine SOX2 gene, crucial for embryonic development. Its conserved sequence and expression in developing gonads suggest a role in sheep germ cell development.
Area of Science:
- Genetics
- Developmental Biology
- Reproductive Biology
Background:
- The SOX gene family encodes transcription factors with a conserved HMG-box DNA-binding domain.
- These genes are critical during embryonic development.
- Understanding SOX2's role in sheep reproduction is important for livestock genetics.
Purpose of the Study:
- To clone and characterize the ovine SOX2 transcript.
- To investigate the expression pattern of ovine SOX2 in developing gonads.
- To determine the evolutionary conservation and chromosomal location of ovine SOX2.
Main Methods:
- Screening of a sheep testis (12 days post-coitum) cDNA library using an SRY-HMG-box probe.
- 3' RACE (Rapid Amplification of cDNA Ends) experiments.
- Sequence comparison with human, mouse, and chicken SOX2 homologues.
- Chromosomal localization analysis.
Main Results:
- The ovine SOX2 transcript was successfully cloned and characterized.
- Ovine SOX2 sequence shows strong conservation with orthologs in other species.
- Ovine SOX2 is located on sheep chromosome 1q33.
- SOX2 expression was observed in developing gonads, indicating its involvement in germ cell development.
Conclusions:
- Ovine SOX2 is a highly conserved gene with a specific chromosomal location.
- The expression pattern supports the hypothesis that SOX2 plays a role in the sheep germ cell line.
- This finding contributes to understanding the genetic regulation of reproduction in sheep.