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Published on: March 8, 2012
Characterization of an HIV-1 p24gag epitope recognized by a CD8+ cytotoxic T-cell clone
F Buseyne1, S Stevanovic, H G Rammensee
1Unité de virologie et d'immunologie cellulaire, URA CNRS 1157, Institut Pasteur, Paris, France.
Abstract:
A CD8+ cytotoxic T-cell clone that recognized HIV p24gag was isolated from an infected individual. The minimal epitope was localized to amino acids 308-316 (QASQEVKNW). Using allogeneic target cells, we found that lysis was restricted by the HLA-Cw0401 molecule. We observed that C1R cells, that express the HLA-Cw0401 allele are able to present the peptide to the cytotoxic clone, but with reduced efficiency. Other B-cell lines, that have been genotyped as HLA-Cw0401+ were unable to present the peptide to the clone, suggesting the existence of other variants of HLA-Cw0401 or a loss of cell surface expression of this molecule.
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