Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of Bacteroides forsythus isolates

T Takemoto1, H Kurihara, G Dahlen

  • 1Department of Endodontology and Periodontology, Hiroshima University School of Dentistry, Japan.

Journal of Clinical Microbiology
|June 1, 1997
PubMed
Summary

Bacteroides forsythus, a key periodontitis bacterium, showed high virulence when combined with Porphyromonas gingivalis in rabbit models. This coinfection led to abscesses and sepsis, highlighting the synergistic pathogenicity of these oral bacteria.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibiotic resistance among Aerobic Gram-Negative Bacilli isolated from patients with oral inflammatory dysbiotic conditions-a retrospective study.

Frontiers in dental medicine·2025
Same author

Low prevalence of multi-resistant bacteria in undergraduate dental students; an observational case-control multi-centre study in Europe.

Journal of oral microbiology·2021
Same author

Immediate clinical and microbiological evaluation of the effectiveness of 0.5% versus 3% sodium hypochlorite in root canal treatment: A quasi-randomized controlled trial.

International endodontic journal·2019
Same author

Salivary microbial profiles in 5-year old children with oral clefts: a comparative study.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2018
Same author

Dentistry in crisis: time to change. La Cascada Declaration.

Australian dental journal·2017
Same author

Low antibiotic resistance among anaerobic Gram-negative bacteria in periodontitis 5 years following metronidazole therapy.

Anaerobe·2016

Area of Science:

  • Microbiology
  • Periodontology
  • Bacterial Pathogenesis

Background:

  • Periodontitis is a complex oral infection often involving polymicrobial communities.
  • Bacteroides forsythus is frequently isolated from periodontitis lesions, but its pathogenic role requires further elucidation.

Purpose of the Study:

  • To characterize clinical isolates of Bacteroides forsythus.
  • To evaluate the pathogenicity of B. forsythus, alone and in combination with other oral bacteria, using a rabbit wound chamber model.

Main Methods:

  • Characterization included growth studies, enzymatic and antibiotic susceptibility profiling, 16S rRNA ribotyping, and SDS-PAGE of outer membrane proteins.
  • Pathogenicity was assessed via monoinoculation and coinoculation with Fusobacterium nucleatum or Porphyromonas gingivalis in a rabbit wound chamber model.

Related Experiment Videos

Main Results:

  • All B. forsythus strains exhibited trypsin-like activity and sensitivity to most tested antibiotics, except ciprofloxacin.
  • Monoinoculation did not cause abscesses, but coinoculation with F. nucleatum or P. gingivalis resulted in abscess formation in 75% and 100% of rabbits, respectively.
  • Coinfection with P. gingivalis led to sepsis and mortality in rabbits, with B. forsythus demonstrating high virulence and invasiveness.

Conclusions:

  • Bacteroides forsythus strains possess specific characteristics and exhibit synergistic virulence with P. gingivalis.
  • The combination of B. forsythus and P. gingivalis significantly enhances pathogenicity, leading to severe outcomes in a relevant animal model.