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Propagation of antigen-specific T cell helper function in vitro
Journal of Immunology (Baltimore, Md. : 1950)
|December 1, 1979
Summary
T cell proliferation and antibody production require Ly-1 positive cells. Antigen-specific helper T cells were enriched, demonstrating Ly-1 dependence for immune responses.
Area of Science:
- Immunology
- Cell Biology
- T cell immunology
Background:
- Lymph node cells play a crucial role in adaptive immunity.
- T cell subsets mediate distinct immune functions, including helper activity and proliferation.
- Understanding T cell subset roles is vital for dissecting immune responses.
Purpose of the Study:
- To investigate the role of Ly-1 and Ly-2 T cell subsets in antigen-induced proliferation.
- To characterize the helper activity of enriched T cells in antibody production.
- To elucidate the requirements for antigen-specific T cell responses.
Main Methods:
- Primed lymph node cells were treated with anti-Ly 1 or anti-Ly 2 serum and complement.
- T cells were activated with antigen in vitro and propagated in antigen-free supernatant.
- Helper activity was measured via a secondary hapten-carrier response.
- Antigen-induced proliferative response was assessed in the presence of filler cells.
Main Results:
- Treatment with anti-Ly 1 serum and complement abrogated antigen-induced proliferation.
- Anti-Ly 2 serum and complement did not affect proliferation.
- Enriched T cells showed stepwise enhancement of helper activity for antibody production.
- Increased helper activity correlated with enhanced antigen-induced proliferation, dependent on filler cells.
Conclusions:
- Ly-1 positive T cells are essential for antigen-induced proliferation.
- Ly-1 positive T cells possess helper activity crucial for antibody production.
- Antigen-specific T cell responses, including proliferation and help, are mediated by Ly-1+ T cells.