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Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit
M Woodhead1, M A Taylor, H V Davies
1Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Dundee, Scotland, UK.
Molecular Biotechnology
|February 1, 1997
Abstract:
Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction.