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Isolation and characterization of a processed pseudogene for murine cyclin D3
1Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
Molecules and Cells
|April 30, 1997
Summary
Researchers identified a murine cyclin D3 pseudogene, a non-functional gene copy. This pseudogene, estimated to be 11 million years old, offers insights into gene evolution.
Area of Science:
- Genetics
- Molecular Biology
- Evolutionary Biology
Background:
- Cyclin D3 is a key regulator of the cell cycle.
- Pseudogenes are non-functional copies of genes that can provide evolutionary insights.
Purpose of the Study:
- To characterize the murine cyclin D3 pseudogene.
- To determine the evolutionary age of the cyclin D3 pseudogene.
Main Methods:
- Isolation of genomic clones using radiolabeled murine cyclin D3 cDNA probe.
- Physical mapping and DNA sequence analysis.
- Comparison of pseudogene and cDNA sequences.
Main Results:
- Two cyclin D3 genomic clones (MCD3P-117 and MCD3P-327) were isolated, containing a 1.5 kb processed pseudogene.
- The pseudogene shares 90% sequence identity with cyclin D3 cDNA but contains genetic lesions and mutations.
- The cyclin D3 pseudogene is estimated to have arisen approximately 11 million years ago.
Conclusions:
- This study provides the first characterization of a murine cyclin D3 pseudogene.
- The findings offer insights into the evolutionary history and age of the cyclin D3 pseudogene.
