Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acid, protons and Helicobacter pylori

G Sachs1, K Meyer-Rosberg, D R Scott

  • 1Department of Medicine and Physiology, University of California, USA.

The Yale Journal of Biology and Medicine
|May 1, 1996
PubMed
Summary

Proton pump inhibitors effectively treat acid-related disorders and enhance antibiotic efficacy for Helicobacter pylori eradication. Urease activity in H. pylori maintains proton motive force in acidic environments, explaining its survival and synergistic treatment outcomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A luminous fast radio burst that probes the Universe at redshift 1.

Science (New York, N.Y.)·2023
Same author

A census of baryons in the Universe from localized fast radio bursts.

Nature·2020
Same author

Letter: vonoprazan, a long-lasting acid suppressor of the gastric H+, K+-ATPases with - implications for renal H+, K+-ATPases; authors' reply.

Alimentary pharmacology & therapeutics·2016
Same author

Corrigendum. The binding selectivity of vonoprazan (TAK-438) to the gastric H+,K+-ATPase.

Alimentary pharmacology & therapeutics·2016
Same author

The binding selectivity of vonoprazan (TAK-438) to the gastric H+, K+ -ATPase.

Alimentary pharmacology & therapeutics·2015
Same author

Colloidal bismuth subcitrate impedes proton entry into Helicobacter pylori and increases the efficacy of growth-dependent antibiotics.

Alimentary pharmacology & therapeutics·2015

Area of Science:

  • Gastroenterology
  • Microbiology
  • Pharmacology

Background:

  • Proton pump inhibitors (PPIs) target gastric H+/K+ ATPase, effectively inhibiting acid secretion for reflux and peptic ulcer disease.
  • Clinical data indicate that Helicobacter pylori eradication is crucial alongside lesion treatment to prevent recurrence.
  • PPIs demonstrate synergistic effects with antibiotics for H. pylori eradication.

Purpose of the Study:

  • To investigate the mechanism of H. pylori survival in the gastric environment.
  • To understand the role of urease activity and proton motive force (pmf) in H. pylori acid tolerance.
  • To elucidate the basis for the synergistic effect of PPIs and antibiotics in H. pylori eradication.

Main Methods:

  • Measurement of intracellular pH and membrane potential of H. pylori using fluorescent dye probes (BCECF, DiSC3) at varying external pH.

Related Experiment Videos

  • Calculation of proton motive force (pmf) under different pH conditions.
  • Assessment of the effect of urea on pmf and bacterial survival in simulated gastric environments.

    • Main Results:

    • H. pylori maintains a significant pmf (-215 mV at pH 7.0) composed of delta pH and membrane potential gradients.
    • Bacterial survival at different external pH levels correlates with the ability to maintain pmf.
    • Urea addition restored pmf at acidic pH (3.0-3.5) but abolished it at neutral/alkaline pH (≥7.0) due to ammonia production.
    • Urease activity enables H. pylori to tolerate acidic conditions but also limits its survival in highly acidic environments.

    Conclusions:

    • H. pylori is an acid-tolerant neutrophile, with urease activity being key to its survival in the stomach.
    • The ability to maintain pmf is critical for H. pylori survival.
    • PPI-induced alterations in gastric pH and distribution of H. pylori contribute to the observed synergism with antibiotics for eradication.