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Prenatal analysis of rhesus CcDEe blood groups by heteroduplex generator
N C Rose1, C Hurwitz, L Silberstein
1Department of Obstetrics and Gynecology, University of Pennsylvania Medical Center, Philadelphia, USA.
Objective:
Our purpose was to determine the efficacy of the heteroduplex generator in the prenatal analysis of rhesus CcDEe blood groups.
Study Design:
A cross-sectional study was performed evaluating fetal samples from 85 women undergoing prenatal diagnosis and comparing the results with standard immunologic serotyping on cord blood delivery.
Results:
Of the 85 samples, 64 were tested, for all CcDEe alleles: one case was discrepant. Twenty-one cases were tested solely for the D antigen. Two novel genotypes were detected in the population by heteroduplex generator and confirmed by deoxyribonucleic acid sequencing. Five cases were indeterminate because of an indistinct banding pattern.
Conclusions:
Heteroduplex analysis can identify rhesus blood group alleles and is inexpensive, rapid, and does not use radioactive isotopes.